Characterized by its generous packaging capacity, the recombinant lentivirus ensures robust and enduring trans gene expression. Its ability to integrate into the host genome facilitates long-term gene expression. Additionally, the lentivirus efficiently transduce a wide variety of mammalian cell lines, including primary and stem cells, even those traditionally challenging to transfect.
Lentivirus packaging service details:
- For our work, we employed the VSV-G pseudotyped second-generation lentivirus.
- Lentivirus particles purified using sucrose garden ultracentrifugation
- To determine viral titers, we utilized the Lenny-X GoStix Plus instant titration system (for crude lysate) and p24 ELISA-based protocol (for ultra-purified large scale packaging).
| Scale | Application | Titer and volume | Price | Turnaround* |
|---|---|---|---|---|
| Non-ultra-purified (Crude lysate) | Cell culture | 1E8 IFU/ml, 500 ul | $532 | 2 weeks |
| Ultra-purified Large scale | Cell culture and in vivo | 1E9 IFU/ml, 250 ul | $1152 | 3 weeks |
IFU- infectious units
*Estimated turnaround time may vary depending on the number of jobs queue ahead of yours.
Shipping and storage
Lentivirus is stored in HBSS buffer. The shipments packed with dry ice to maintain stability. Upon, receipt, it is advisable to store the lentivirus at -80oC fro long-term storage, ensuring its stability for at least one year. For short-term storage, such as 1-2 weeks, -20oC is suitable. Avoid repeated freeze-thaw cycles.
Safety
Lentivirus is categorized under biosafety level 2 (BSL-2).