FAQs

  Cloning DNA prep Packaging only

AAV

 5-10 ug at conc. of >0.5 ug/ul 1 ug

Small-scale AAV>150 ug;

Large-scale AAV>300 ug;
Adenovirus 5-10 ug at conc. of >0.5 ug/ul 1 ug 5-10 ug at conc. of >0.5 ug/ul
Lentivirus 5-10 ug at conc. of >0.5 ug/ul 1 ug

Small-scale AAV>150 ug;

Large-scale AAV>300 ug;

We strongly recommend sending DNA obtained from endotoxin-free maxiprep.

We require the following information: 

  1. Plasmid name
  2. DNA concentration of plasmid (ug/ul)
  3. Sequence/map of plasmid

Avoid repeating freeze-thaw cycles. Store the viral stock immediately upon arrival. Aliquot right before use and keep at -80C for long term storage.

Viral Production Core guarantees that the packaged virus will contain the customer's insert at the designated titer. If the designated titer is not achieved after one attempt, we will repeat the AAV service a second time at no cost to the customer. If the designated titer is not obtained after the second attempt, we will contact the customer to discuss the project and potential options before proceeding. Viral Production Core can provide additional services to check the virus expression level after purification. Additional charges may be apply.

Characteristic AVV Adenovirus Lentivirus
Genome 4.8 kd (ssDNA) 36 kd (dsDNA)  9 kb (ssRNA) 
Parckaging Capacity 4.7 kb 7.5 kb

6 kb
Infection Most dividing and non-dividing cells
Transduction Efficiency Moderate High Moderate
Integrating Non-integrating Non-integrating Integrating
Expression Transient or stable Transient Integrating
Immunogenicity Very Low High  Low
Biosafety BSL-1 BSL-2 BSL-2

Viral Production Core is excited to begin using iLab online system. To register or log-in, please, navigate to the https://ua.ilab.agilent.com/landing/3645 and click on the green "login" button at the top right of the screen. New customers who do not have an iLab account, please email ilab-support@agilent.com to assist with registration.