Recombinant adenovirus vectors offer high transgene expression in various mammalian cell types without integration into the host genome. Their efficiency and short-term gene expression make them a preferred tool for in vivo gene delivery.
Our core ensure superior titer, purity, viability, and consistency.
Adenovirus packaging service details:
- We provide human Ad5 adenovirus type.
- Our system employs replication-incompetent adenovirus.
- Adenovirus particles are purified using CsCl gradient ultracentrifugation.
- Viral titer is analyzed by measuring optical density ( using OD260) of the viral particles.
| Scale | Application | Titer and Volume | Price | Turnaround* |
|---|---|---|---|---|
| Ultra-purified Midi | Cell culture and in vivo | 1E9 PFU/ml, 500ul | $1151 | 3-4 weeks |
| Ultra-purified Maxi | Cell culture and in vivo | 1E9 PFU/ml, 1000ul | $1719 | 3-4 weeks |
PFU- Plaque formation units
*The estimated timeline depends on the number of jobs in queue ahead of yours.
Adenovirus primary stock (recombination and amplification) service details
The construction of the adenovirus was accomplished using the pAdEasy system, utilizing homologous recombination in bacteria.
The process involved the following steps:
- Transferring the expression cassette into the adenovirus vector.
- Generating the first generation of low-titer virus stock.
| Application | Titer and volume | Price | Turnaround | |
|---|---|---|---|---|
| Adenovirus primary stock | Cell culture | 1E8 PFU/ml, 1000 ul | $955 | 4-5 weeks |
In case the adenovirus vector encodes a fluorescent protein, we will conduct a transduction test to detect the corresponding fluorescence.
Shipping and storage
Adenovirus is stored in HBSS buffer. The adenovirus shipments packed with dry ice to maintain stability. Upon, receipt, it is advisable to store the adenovirus at -80oC fro long-term storage, ensuring its stability for at least one year. For short-term storage, such as 2-3 weeks, -20oC is suitable. Once thawed, the adenovirus can be stored at 4oC for 1-2 weeks. Avoid repeated freeze-thaw cycles.
Safety
Our vector's safety is guaranteed by its engineered design, lacking essential genes for virus production. These genes are incorporated into the genome of packaging cells. As a result, the virus is replication incompetent, except when used to transduce packaging cells. This ensures enhanced safety and minimized risk during experiments and applications.